Breast cancer is marked by specific, Public T-cell receptor CDR3 regions shared by mice and humans
Fig 2
Convergent Recombination Dominates the Public Repertoire and the tumor-developing mice repertoire.
Public repertoires and their subtypes are shown in tumor-developing mice and in Control mice using nucleotide sequences (A) and using AA sequences (B) in α chain. The Y-axis in the panels shows the percentages of “unique” clones, where we used the common definition of “unique” sequences as that in which we count each sequence only once and disregard its copy-number. The two panels show the repertoire in the α chain, but a similar effect is seen in the beta chain (S1 Fig). The different categories included in the panel bars are relative abundance of the different categories. That is, together they represent 100% of the sequences. Therefore, the color bars together sum up to 100%. (C, D) Convergent recombination in Control and Transgenic mice in α chain. The upper bars indicate nucleotide (NT) sequences and their division to the different Public groups (Private, Public-Inclusive and public-Exclusive), while the lower bars indicate amino-acid (AA) sequences in Control mice (C) and tumor-developing mice (D). The lines between NT and AA represent the effect we see in convergent recombination–in which different NT sequences encode to the same AA sequence and change the Public/Private balance. For example, in panel C, we can see that 5% of the NT sequences that were Private in an NT view, became 66% of the Public Exclusive when we looked in an AA view. (E, F) Frequencies of CR clones in the Public repertoire in α and β chains. (G, H) Correlation between the averaged mouse-mouse sharing level and CR level in Control versus tumor-developing groups (G) and in the Early-tumor group versus Cancer group (H) in β chain.