Breast cancer is marked by specific, Public T-cell receptor CDR3 regions shared by mice and humans
Fig 1
(A) 120 blood samples were drawn from the retro-orbital sinus of 10 FVB/N-Tg(MMTVneu), a mouse model of HER2 human breast cancer mice, and from 5 FVB/NJ Control mice. Over these 8 time-points, none of the Control mice (blue) developed any tumors. We will use the term "Transgenic" for FVB/N-Tg(MMTVneu) 202 Mul/J and "Control" for FVB/NJ strain. Progress of tumor in the ten Transgenic mice is demonstrated using the red colored samples in the figure. The last time point before tumors are shown was defined as pre-cancer and marked light red. (B) At each time point, the peripheral blood mononuclear cells were isolated and stained for flow cytometry. Cells were analyzed and gated for sorting using a FACS ARIA III sorter, and the CD4+CD62LhiCD44lo naïve population was separated for RNA extraction and T cell receptor library preparation (see Methods). TCR alpha and TCR beta were obtained separately and not via single-cell TCR sequencing (alpha-beta pairing is not possible).