Practical fluorescence reconstruction microscopy for large samples and low-magnification imaging
Fig 4
Coarse-to-fine feature reconstruction.
(A) A representative transmitted-light image of HUVEC cells at 20x magnification with its corresponding structures of varying scale (B-D). The scale bar represents 30 μm. (B-D) the relatively large nuclei, the finer VE-Cadherin structures, and thin F-Actin filaments which are not readily resolved by the network. Ground truth fluorescent features are displayed alongside, and merged with, network predictions. (E) displays zoomed-in portions of images shown in (D). Line sections from (B”, C”, and D”) are displayed graphically in (B”‘, C”‘, D”‘), to enable intensity comparisons across the ground truth and predicted features. (F) summarizes the distribution statistics, clearly showing the uncertainty in F-actin, with tighter reconstruction for nuclei and VE-cadherin. N = 5500+ test images for these datasets.