Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples
Fig 7
Validation of the predicted FOXM1 subnetwork.
(a) The subnetwork of FOXM1 with predicted target genes. (b) Validation of the expression changes of the predicted target genes of FOXM1 with perturbation experiments. MCF-7 cells were treated with DMSO (control) or thiostrepton (a FOXM1 inhibitor) for 48 hours. Except for SCCBP1 and STIL, the other 6 genes were significantly down-regulated after FOXM1 inhibition. (c-e) ChIP-seq analysis of FOXM1 in the MCF-7 cell line with four biological replicates, showing that FOXM1 binds ASPM, CDCA8 and KIF2C. (f-h) ChIP-seq analysis of FOXM1 in the MDA-MB-231 cell line with two biological replicates, showing that FOXM1 binds ASPM, CDCA8 and KIF2C.