Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples
Fig 5
Experimental validation of the predicted role of ACSS1 in EMT of bladder cancer.
(a-b) Expression levels of ACSS1 and CDH1 in 5637 cells when ACSS1 was overexpressed (a) and inhibited (b), measured by q-PCR. (c) Protein expression levels of ACSS1 and CDH1 in 5637 cells when ACSS1 was overexpressed or inhibited, measured by Western-blotting. (d) Quantification of the relative protein expressions. (e) Examples of immunohistochemical expression of ACSS1 and E-cadherin in conventional UC and SARC. Statistical significance was assessed by student’s t test. **P<0.01; ***P<0.001; ****P<0.0001. OE-ACSS1: overexpression of ACSS1; si-NC: small interfering RNA negative control; si-ACSS1: small interfering RNA targeting ACSS1.