Fibrillarin evolution through the Tree of Life: Comparative genomics and microsynteny network analyses provide new insights into the evolutionary history of Fibrillarin
Fig 7
Evolutionary differences between the two microsynteny mammalian clusters.
a) Violin plot of the number of exons in each of the five major clades of Chordata animals and arthropods. Points on the plot represent specific data. The number of exons in Actinopterygii, amphibians, and reptiles ranges from 7–10 exons, while that in mammals ranges from 1–10. The curved arrow above the mammalian violin plot indicates the two microsynteny clusters in figure b. b) Two microsynteny clusters detected in mammals and belonging to FIB (cluster A) and FIB-like genes (cluster B) specifically. Arrows under clusters indicate specific boxplots in figure c. c) Boxplot of the number of exons of the genes from the specific microsynteny clusters of mammals. Genes from cluster A (FIB genes) have a mean of 9 exons, while those in microsynteny cluster B (FIB-like genes) have a mean of one exon per gene. d) Depiction of microsynteny communities on a phylogenetic gene tree of animals. The pairwise syntenic relationship of clusters A and B (FIB and FIB-like, respectively) are indicated with black arrows, and the links are colored following Fig 5 to show the absence of syntenic relationship in both clusters (evolving from different genomic context). Green and yellow lines on the tree represent syntenic pairwise connections. e) Ks values for each microsynteny cluster. For the analysis, we carried 1800 and 2664 comparisons of homologous proteins for clusters A and B, respectively. f) Genes chosen from each syntenic cluster were inspected for expression values from transcriptomic atlases (as described in materials and methods). Colored nodes within clusters represent the genes chosen for the analysis. We use genes from species that had two copies, one in each cluster (one FIB and one FIB-like gene), and that had expression information available in the Expression Atlas (EMBL-EBI). g) Heatmap from the expression values of chosen genes in f, clustered according to taxonomy. On the "x" axis (*)frontal lobe, and (**)lung are the tissues used in the analysis.