Graph-theoretical formulation of the generalized epitope-based vaccine design problem
Fig 7
Mosaic vaccines naturally target conserved regions even when this is not required.
(a) shows, for each residue position in aligned sequences where the consensus is not a gap, the smoothed entropy (blue, and residue entropy in lighter color) and the potential immunogenicity (green) (b) shows the number of pathogens covered in each position by a 20-epitopes mixture with maximal immunogenicity (yellow), a short mosaic of 28 amino acids (red) and a long mosaic of 90 amino acids (blue). The count is normalized separately for each vaccine to account for their different coverage. (c) shows the pairwise correlations of the variables shown in the left plot, so that every dot in the scatter plots corresponds to a different residue position, and linear fits are shown in red. The lower triangular half shows the Spearman correlation coefficients (above) and the respective p-value (below). Colors range from blue (large negative correlation) to white (no correlation) to red (large positive correlation), and the font is bold if the correlation is significant with a confidence of at least 99.5% at the Bonferroni-corrected significance level of 5%. The diagonal contains histograms showing the distribution of each variable, with logarithmic y axis.