A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology
Fig 6
Population decoding differs in sensitivity and temporal profile between imaging and ephys.
A. Performance of instantaneous regularized linear-discriminant-analysis (LDA) trail-type decoder for 100-unit subpopulations. Vertical dotted lines indicate behavioral epochs, from left to right: presample, sample, delay, response. Top, decoders trained on ephys; middle, decoders trained on 6s-AAV; bottom, difference between the two. For top and bottom plots: individual gray lines show single subsample performance and black thick line shows average. In bottom plot mean is indicated by think line and shaded area corresponds to standard deviation. B. Toy model demonstrating observed delayed but enhanced decodability in imaging data. Schematic of relation between activity (left) and decodability (right) when the model has two constant levels of activation for the two trial types (orange and red). C. Example cell showing similar behavior to the toy model. D. Comparison of decodability from imaging to decodability from 1-second filtered ephys. Top, 1-second filtered ephys; bottom, difference between filtered ephys and imaging. E. Comparison of decodability of trial type per behavioral epoch. Decodability for all datasets separated into slow indicators (left) and fast indicators (right). Bars color coded according to dataset. Left: black, ephys; magenta, 6s-AAV; red, 6s-TG; green, 6s-AAV synthetic; cyan, 6s-TG synthetic. Right: black, ephys (depth matched to 6f-TG); orange, 6f-TG; purple, 6f-TG synthetic. F. Accuracy of trial-type population decoding over time for different datasets. Left, top to bottom: 6s-TG, 6s-AAV, 6f-TG. Middle: ephys. Right, accuracy of trial-type population decoding over time of datasets comprised of inferred ephys from the different imaging datasets. top to bottom: 6s-TG, 6s-AAV, 6f-TG. Left column: MCMC framework, right column: MLSpike framework. G-I. Performance of behavioral-epoch LDA decoders. G. Probability of decoder based on ephys to assign population activity to each of the different epochs shown in the following color scheme: pre-sample (blue), sample (orange), delay (green), and response (red) epoch; arrows indicate the inferred transition times of epochs from neural codes. H. Same plot format as G, but for imaging. I. Sample plot format as G, but for synthetic imaging.