A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology
Fig 5
Different sources of variability extracted in dimensionality reduction on imaging and ephys.
A. Fraction of variance of neural activity explained by principal components 1–10 divided into different sources of variability: red: temporal dynamics; blue: trial type; yellow: other (interaction term). From left to right: ephys, 6s-AAV, 6s-TG, 6s-AAV synthetic, 6s-TG synthetic; ephys depth-matched to 6f-TG recordings, 6f-TG, 6f-TG synthetic. Vertical dashed line indicates the PC index at which the remaining components capture <1% of total variance. B. Trial-averaged scores of first three PCs over time (from top to bottom), averaged separately for the two trial types (right trial, blue; left trial, red). Same order from left to right as in A. C. Trial dynamics in the first two-PC subspace for the two trial types (right trial, blue; left trial, red). Same order from left to right as in A. D. Left: fraction of variance explained by principal components 1–3 for each of the datasets, and its division into different sources of variability: red: temporal dynamics; blue: trial type; yellow: other (interaction term). Bars from left to right: ephys, 6s-TG, 6s-AAV; ephys depth-matched to 6f-TG recordings, 6f-TG. Middle: equivalent results for principal component analysis performed on inferred spiking data obtained via the MCMC framework. Right: equivalent results for principal component analysis performed on inferred spiking data obtained via the MLSpike framework.