A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology
Fig 3
Simultaneous loose-seal recordings and calcium imaging of layer 2/3 pyramidal neurons in vivo.
A. Illustration of the recording setup. Transgenic mice expressing GCaMP6s (GP4.3) or GCaMP6f (GP5.17) were lightly anesthetized and viewed drifting grating visual stimuli. GCaMP-expressing L2/3 neurons were recorded in the loose-seal mode during calcium imaging. B. Example recordings from neurons expressing GCaMP6f (top, 6f-TG) and GCaMP6s (bottom, 6s-TG). Red ticks, spikes. C. Traces of fluorescence dynamics following different numbers of action potentials (APs) for example neurons. Top, 6f-TG; bottom, 6s-TG. Gray, no AP; black, a single AP; red, 2 APs; blue, 3APs; green, 4APs; magenta, 5APs. Thin lines, single trials; thick lines, average. D. Peak fluorescence increases as a function of the number of spikes in 200 ms bins. Black, single trials; red, trial average. E. ROC curve of all spike events. Inner panel, ROC curve for single AP events. F. Distribution of d-prime for single spikes across cells. Left, 6s-TG; right, 6f-TG. G. Mean peak fluorescence changes as a function of number of spikes in 200 ms time intervals across cells. Left, 6s-TG; right, 6f-TG. Each circle corresponds to a recorded neuron. Bars indicate average.