A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology
Fig 2
Single neuron trial-type selectivity differs between imaging and ephys.
A. Example neurons with monophasic selectivity. Left, ephys; right, imaging. B, Same as A for multiphasic neurons. C, Same as A for non-selective neurons. D-F, Fraction of selective neurons in depth-matched ephys (“ephys @6f” indicates depth matched to the more superficial 6f-TG recordings) and when imaged with 6s-AAV, 6s-TG, or 6f-TG. D. Fractions of monophasic neurons. E. Fraction of multiphasic neurons. F. Fraction of nonselective G. Proportion of multiphasic neurons in intracellular recordings is similar to that in extracellular recordings. Bar shows fraction of neurons in each of the categories for extracellular (left) and intracellular (right) ephys. H. Effect of spike inference on estimates of fractions of monophasic (left) and multiphasic (right) neurons. The distribution of fraction of neurons for imaging data (source data), is given in gray for 6s-AAV (top), 6s-TG (middle) and 6f-TG (bottom). The distribution for ephys (target data) is in black. Distributions from inferred spike rates from MCMC (40) are in cyan and for MLSpike (42) are in magenta. Arrows denote the difference between the imaging data and ephys data (gray arrow) or inferred ephys and ephys data (cyan arrow for MCMC and magenta arrow for MLSpike). I. Fraction of right-preferring neurons in the different datasets divided into slow indicators (left) and fast indicators (right). J. Bar plot of fractions of ramp-down, ramp-up and ‘other’ cells in ephys for right-preferring (left) and left-preferring neurons (right).