Modeling microbial cross-feeding at intermediate scale portrays community dynamics and species coexistence
Fig 6
Prediction of the long-term steady state of the community of 14 amino acid auxotrophies.
(A) Parameters other than the amino acid leakage fractions (obtained from fitting pairwise coculture data in Fig 5) were manually optimized from the observed population density during a 7-day 100-fold serial dilution of one 14-auxotroph and four 13-auxotroph communities. Filled circles: experiments [22]; Lines: simulation. (B) Simulation of the trained 14-member model over 50 daily passages of the community into fresh medium. The observed long-term stable coexistence of a four-auxotroph subset (ΔI, ΔK, ΔM, ΔT) was correctly predicted. The two replicates of experimental observations were adopted from Mee et al. [22]. See Fig 5 legend for abbreviations of the names of amino acid auxotrophies.