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In silico co-factor balance estimation using constraint-based modelling informs metabolic engineering in Escherichia coli

Table 2

Maximum yield (Y^E and Y^Ea), pathway yield (Y^P), adjusted pathway yields (Y^P,G and Y^P,G,X) and pathway efficiency (η) of all butanol and butanol precursor pathways calculated using the stoichiometric and energetic calculations proposed by Dugar et al. [5].

Maximum yield (Y^E) is the maximum amount of product that can be produced from the substrate. Y^Ea indicates the maximum yield in mol product/mol substrate. Pathway yield, Y^P, is pathway-specific and calculated from pathway stoichiometry. Y^P,G is the adjusted pathway yield once any excess redox is depleted using an electron sink (i.e. glycerol). Y^P,G,X is the adjusted pathway yield after any excess ATP is diverted towards biomass formation. η is the ratio between Y^P,G,X and Y^E.

doi: https://doi.org/10.1371/journal.pcbi.1008125.t002