In silico co-factor balance estimation using constraint-based modelling informs metabolic engineering in Escherichia coli
Fig 7
butanol carbon yield (%) and biomass production rates (mmol gDW-1hr-1) of engineered E.coli strains in response to changes in ATP and NADH demands.
Each model represents a unique pathway variant for butanol production, which has been manually curated and optimized for the selected objective under aerobic conditions. (A) BuOH-0, comprised of route AtoB + AdhE2; (B) BuOH-1, including reactions NphT7 + AdhE2; (C) tpcBuOH, made up of AtoB + TPC7; (D) fasBuOH, comprising reactions NphT7 + TPC7.