Epithelial organ shape is generated by patterned actomyosin contractility and maintained by the extracellular matrix
Fig 5
Quantitative and qualitative comparisons of experimental data with simulation results for different levels of simulated basal actomyosin contractility.
(A) Initial and final frames of simulation result where actomyosin contractility leads to curved profile shape. (B) Global curvature of apical pouch surface and basal peripodial surface for experimental (n = 16) and simulated (n = 1) wing discs. Experimental data are mean ± standard deviation. (C) Relative nuclei positions of pouch cells. Note, the relative nuclei position has a value of zero at the basal surface and one at the apical surface. (D) Height of pouch cells. In C and D, boxplots show minimum, first quantile, mean, third quartile, maximum and outlier of simulated (n = 65), and experimental (n = 1064) columnar cells. Measurements for columnar cell heights experimentally and computationally were taken as the summation of the length of straight lines from apical to centers of segmented nuclei centers and from segmented nuclei centers to the basal side of the cells. (E) Comparison of local curvature profiles and shapes obtained from experimental data and computational results.