Epithelial organ shape is generated by patterned actomyosin contractility and maintained by the extracellular matrix
Fig 3
Potential mechanisms of wing disc bending.
(A) The patterned ECM tension hypothesis. In this hypothesis, the level of passive tension of the ECM is higher next to the columnar cells compared with ECM next to squamous cells. (A’) Graphical illustration of potential hypothesis explaining how high ECM tension at the basal side of the columnar cells compresses the tissue and contributes to curvature profile of wing disc. (B) The patterned actomyosin contractility hypothesis. The columnar cells were stained for actin by Phalloidin and an antibody to P-Myosin II. (B’) Schematic of hypothesized mechanism for generating epithelial bending and asymmetrical nuclear positioning in the wing imaginal disc. Actomyosin contractility beneath the nucleus of columnar cells drives wing disc bending.