Pseudocell Tracer—A method for inferring dynamic trajectories using scRNAseq and its application to B cells undergoing immunoglobulin class switch recombination
Fig 4
Pseudocell Tracer models IgG1 class switching process.
(A) Pseudocells generated along the IgM to IgG1 axis. Plot of relative expression of Aicda, Ighm and Ighg1 along the IgM to IgG1 axis, where solid line indicates average expression and shading indicates 95% confidence interval. (B) Associative clustering of genes during CSR. Regions of early (left), middle (center), and late (right) transcriptional dynamics are depicted. Plots of relative expression for key genes with specific dynamics, including (C) Stat6, (D) Bach2, (E) Il4ra, and (F) Ifngr1.