Template-based mapping of dynamic motifs in tissue morphogenesis
Fig 4
Dynamics and efficiency of T1-transitions.
(A,B) The first and last snapshots of GBE imaged in the wild-type (A) and bcd nos tsl (B) embryos. Quantification of the average number of T1-transition events within a size normalized tissue of 100 cells throughout GBE shows a significant decrease in their prevalence within the mutant (p = 0.0041). (C) The rates of junction contraction (red) and growth (green) in wild-type embryos. Horizontal axis represents the time before contraction completion and after growth initiation. Vertical dashed lines indicate a time gap of between the two events (1.53±1.48 min, see also Fig 2,3 in S2 File). (D) Same as (C) but for bcd nos tsl embryos (time gap: 1.53±1.7 min). (E) Rose diagrams showing angle distributions of contracting junctions with respect to the ventral midline (i: 91.70±18.30°; p << 0.05 Rayleigh test for circular uniformity), and of growing junctions (ii: 1.20±35.90°; p << 0.05). (F) Same as (E) but in bcd nos tsl embryos (83.90±41.40°; p = 0.011 for contraction; 2.40±41.90°; p = 0.012 for growth). 0° = posterior, 90° = dorsal. (G) Bottom: Manually drawn template for a reversing T1-transition. Middle: An example of an identified reversal. Top: Snapshots of the identified reversal. (H) Probabilities of T1 rearrangements and reversals in the wild-type embryos (based on 539 events). (I) Same as (H) but for bcd nos tsl embryos (based on 60 events).