AlbaTraDIS: Comparative analysis of large datasets from parallel transposon mutagenesis experiments
Fig 1
The underlying method for AlbaTraDIS.
The inputs are insert site plots, with a frequency count of the insertions at each base in the genome for a condition and controls and the annotated genome in EMBL format. The abundance of inserts are normalised and the plots split into forward strand, reverse strand and combined strand insertions. Essentiality and differential abundance is assessed using sliding windows or a per gene option. The height of the log fold change plot indicates the log fold change difference in insertions between the conditions and controls. The list of significant genes is compiled using user definable values of corrected p value (q-value), logCPM (Logarithm Count Per Million) and logFC (Logarithm Fold Change).