Reliable estimation of membrane curvature for cryo-electron tomography
Fig 12
Application of AVV to Golgi and vesicles from cryo-ET.
(A) Two different views of surfaces of Golgi and vesicles (from a primary mouse neuron) generated using the compartment segmentation showing curvedness estimated by AVV with rh of 10 nm. Color scale was set to the value range of [0, 0.1] nm-1 (scale bar: 100 nm). (B) Relative exceedance frequency histograms (reversed cululative histograms with frequency normalized to the total surface area of each compartment) of the curvedness of cER (shown in Fig 11A and 11B), Golgi and vesicles (shown in panel A of this figure), excluding values within 1 nm to surface border. The tomogram and segmentations are deposited in EM Data Bank (EMD-10766).