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Reliable estimation of membrane curvature for cryo-electron tomography

Fig 4

Estimation of normals on a noisy plane.

(A) True normals (black arrows) on a smooth plane surface (yellow). (B) Normals on a noisy plane, where each triangle vertex in the original plane shown in (A) was moved in the direction of its normal vector with Gaussian variance equal to 10% of the average triangle edge. Panels (C-D) show normals on the noisy plane corrected by VV with rh of (C) 4 or (D) 8 voxels. The neighborhoods of a central triangle are shown in a darker yellow. (E) Cumulative relative frequency histogram of normal orientation error for the 10% noisy plane, for initial (uncorrected) normals and those corrected by VV with rh of 4 or 8 voxels. (F) Area under the curve of cumulative relative frequency histograms of normal orientation errors, as shown in (E), for planes with different noise levels. Initial normals and those corrected by VV with rh of 4 or 8 voxels are shown. Curve colors in (E-F) correspond to the colors of the normals in (B-D).

Fig 4

doi: https://doi.org/10.1371/journal.pcbi.1007962.g004