Discriminating between negative cooperativity and ligand binding to independent sites using pre-equilibrium properties of binding curves
Fig 4
(A). Input: θ vs. time for different L, coded in a colorscale. Dashed line indicate equilibrium. (B). θ vs. L at equilibrium fitted with Eq (2) to get ω, K and K10, K01. (C). Time courses simulated with NC model (left) and IB model (right) with the constraints obtained in (B). Only the best fit (dashed lines) is included in each plot. For the example in this figure the data was generated with the IB model (parameters in Table A in S1 Text).