Implications of localized charge for human influenza A H1N1 hemagglutinin evolution: Insights from deep mutational scans
Fig 5
Fitted observed charge in 1918, 1948, 1978, and 2008 computed from fitted per-site multinomial logistic regression models.
These models were fitted using nnet [30] in R [31] (see Materials and methods). All 1744 human HA H1N1 sequences between 1918 and 2008 from fludb [28] were used to fit these statistical models. The arrow is used to highlight sites in the negative branch that are transitioning to positive values.