Implications of localized charge for human influenza A H1N1 hemagglutinin evolution: Insights from deep mutational scans
Fig 3
Evolutionary rates, relative solvent accessibility (RSA), and distances to receptor binding site (RBS) on functional branches.
(A) Normalized conservation scores (labelled rate of evolution) per site overlaid on the functional selection-expected charge plot. These conservation scores were obtained from Rate4site [27] with H1N1 sequences from the fludb database of the IRD [28]. A higher score indicates lower conservation and faster evolution. (B) Empirical cumulative distributions of conservation scores for each of the three branches. (C) Distances to the RBS overlaid on the functional selection-expected charge plot. These distances were calculated using PyMOL [23] (see Materials and methods) (D) Empirical cumulative distributions of the distance to the RBS for each of the three branches. (E) Relative solvent accessibility overlaid on the functional selection-expected charge plot. These scores were computed using dmstools2 [29] (see Materials and methods) (F) Empirical cumulative distributions of relative solvent accessibility for each of the three branches.