Simulating flow induced migration in vascular remodelling
Fig 4
Change in cell shape and density during early stages of development.
A) VE-Cadherin staining of ECs in the yolk sac vasculature of a mouse embryo between 6 and 20 somite stage. Solid yellow line shows the vessel diameter and the dotted yellow line shows the vessel centreline. B) Length and width of the selected ECs shown by yellow arrowed lines whereas green shows an individual EC outline. C) EC length increases between somites 10 and 14. D) No significant change in EC width occurs. E) No difference in the extent of elongation between ECs in vessels with different diameters is present (only data from 20 somites is shown). Each data point represents the average for an embryo (n = 3-4 embryos per stage), with between two and 19 cells per embryo analysed. Values are mean ± SEM. Significance is calculated by one-way ANOVA with Tukey’s test. *P<0.05, and ** <0.01. Scale bar = 50 μm.