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Balance between asymmetry and abundance in multi-domain DNA-binding proteins may regulate the kinetics of their binding to DNA

Fig 4

Percentage of non-specific and specific binding asymmetry in zinc finger proteins (ZFPs) of different lengths.

Each dot represents a ZFP: the average percentage of asymmetric zinc finger (ZF) pairs for each length (i.e., number of ZF domains) is shown by a solid circle, with the corresponding standard deviation shown by the error bar. (A) Asymmetrical non-specific binding was considered to occur when the difference in net charge between two neighbouring ZF domains was σnonspec≥3e. (B) As a course approximation, asymmetrical specific binding was considered to occur when the difference in specificity score between two neighbouring ZF domains was σspec≥0.2.

Fig 4

doi: https://doi.org/10.1371/journal.pcbi.1007867.g004