Computation of condition-dependent proteome allocation reveals variability in the macro and micro nutrient requirements for growth
Fig 6
Computed growth rate in auxotrophic models of iJL1678b when the availability of the essential cofactor in the legend is limited.
For each of the 7 metabolites shown in the legend, reactions were imposed into the model creating an auxotrophy for that metabolite (Table C in S1 Text). Top panel: The growth rate is plotted as a function of the availability of the metabolite indicated in the legend. The percent change in the growth rate compared to the wild-type (prototroph) model is shown. Middle panel: Model-predicted metabolic changes in response to tetrahydrofolate limitation. The mass fraction of protein allocated to each metabolic subsystem during tetrahydrofolate limitation is shown (columns) Bottom panel: Heatmap showing fraction of maximum growth rate-normalized reaction fluxes. The 15 reactions with the highest standard deviation are shown and are highlighted in red if the reaction relies on folate activity. If reaction fluxes were perfectly correlated throughout the tetrahydrofolate limitation simulations, then these reactions were grouped together. The number in parentheses shows the number of other reactions represented by the row. The right column depicts a simulation with the highest folate availability and the left column depicts a simulation with the lowest folate availability.