An in-silico human cell model reveals the influence of spatial organization on RNA splicing
Fig 3
Spliceosomal particle formation depends on NPC count.
Increase (pink) or decrease (blue) in the number NPCs by 20% results in a corresponding change in the number of U1 (A) and U2 (B) particles formed. The effect is consistent for different nuclear sizes. P-values for U1 results are: 1.8 × 10−2 (3.7 μm), 6.8 × 10−3 (4.7 μm), 4 × 10−3 (5.3 μm); and for U2 results are: 3.9 × 10−12 (3.7 μm), 6.4 × 10−6 (4.7 μm), 8.5 × 10−5 (5.3 μm). Error bars represent the standard deviations. For each condition, 20 simulation replicates for duration of 30 seconds were performed.