The influence of red blood cell deformability on hematocrit profiles and platelet margination
Fig 2
Fluorescence stained platelet confocal microscope distributions along the 100μm height axis of an ibidi (Gräfelfing, Germany) glass channel.
10% of platelets were stained with Allophycocyanin (APC) anti-human CD41/CD61 which was excited by a λ = 633nm laser and has a fluorescence emission peak at wavelength λ = 660nm. (A) Image of the stained platelets at the wall (channel height of 96μm). (B) Schematic of the experimental setup of the confocal microscope with the ibidi glass channel and the flowing blood with fluoresces stained platelets. (C) The left panel shows the raw platelet distributions (brown is measured from the 100% healthy case and the purple is the 100% 1.0mM TBHP case). (C) The right panel shows the normalized distributions corrected for absorbance per depth for both 30% hematocrit healthy blood and 30% 1.0mM TBHP treated blood.