Comprehensive analysis of structural and sequencing data reveals almost unconstrained chain pairing in TCRαβ complex
Fig 6
Log-likelihood (LL) distributions for TCRαβ pairs with known antigen specificity.
Cumulative distribution functions of αβ pair LLs computed according to the model shown in Fig 4A. The plot shows n = 1388 real TCRαβ pairs from the VDJdb database (red curves), as well as pairs shuffled within groups specific to the same epitope (orange curves) and pairs shuffled across the entire dataset (dashed black curve). For the latter case, n = 10,000 pairs were selected at random for each epitope pair with re-sampling allowed in order to balance the dataset. Labels above panels are the cognate epitope sequences. Significant differences (Kolmogorov-Smirnov test P-value less than 0.05) between real and shuffled distributions are observed for CINGVCWTV (Kolmogorov-Smirnov test D-value = 0.25, P = 6x10-4), ELAGIGILTV (D = 0.23, P = 2x10-2), GILGFVFTL (D = 0.45, P < 10−15) and GLCTLVAML epitopes (D = 0.26, P = 3x10-8).