Comprehensive analysis of structural and sequencing data reveals almost unconstrained chain pairing in TCRαβ complex
Fig 3
Amino acid statistics at contacting residues.
A two dimensional density plot comparing the number of times a given amino acid combination is observed at an inter-chain contact versus the number of times it was expected to be observed nE. The expected count nE is calculated using amino acid frequency distributions at separate chains and assuming random amino acid pairing; higher nO / nE ratio suggests enrichment of a given amino acid pair at corresponding contacting residues. The number of contacting residue pairs observed with certain nO and nE values (density of points at a given bin) is highlighted by color. Dotted lines show 95% confidence interval for the nO / nE ratio assuming Normal distribution with standard deviation computed from Binomial distribution approximated by nE and the total number of observations nT ≈ 3x105.