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Physicochemical and metabolic constraints for thermodynamics-based stoichiometric modelling under mesophilic growth conditions

Fig 2

Anaplerotic node for E. coli.

Set of carboxylation/decarboxylation reactions including phosphoenolpyruvate, pyruvate, oxaloacetate, and malate. Arrows indicate the expected direction of carbon fluxes. Boxes refer to reactions: blue when they are defined in both the GSM and the metabolic network used for 13C-MFA, and orange when they are exclusively considered in the GSM. In the latter case no mapping was possible (S1 Table).

Fig 2

doi: https://doi.org/10.1371/journal.pcbi.1007694.g002