Methods detecting rhythmic gene expression are biologically relevant only for strong signal
Fig 5
Signal of evolutionary conservation of rhythmic gene expression.
Orthologous genes detected as rhythmic in the same organ of two species have a stronger statistical signal of rhythmicity than those not detected as rhythmic in at least one species. a) Mouse and zebrafish share orthologous genes, some of which are rhythmic in the homologous tissues. b) Method used for ortholog benchmarking, as in panel d: From all mouse genes, only mouse-zebrafish one-to-one orthologs are kept. Considering the liver, these orthologs are separated into two groups: genes for which the ortholog is detected as rhythmic in zebrafish liver, called rhythmic orthologs; and the remaining one-to-one orthologs. c) Chart providing the legends to inform about the method and the threshold used to call genes rhythmic for each condition (species and tissue). d) p-values density distribution of rhythmic orthologs vs non-rhythmic orthologs obtained for the seven methods applied to mouse liver data. Mouse-zebrafish orthologs, that are detected rhythmic in zebrafish liver, are significantly more enriched in small p-values in mouse liver, for all methods (Kolmogorov-Smirnov test p-values < 0.001). Images credit: Anthony Caravaggi (mouse), Ian Quigley (zebrafish).