Providing new insights on the biphasic lifestyle of the predatory bacterium Bdellovibrio bacteriovorus through genome-scale metabolic modeling
Fig 4
Evaluation of the metabolic capabilities of iCH457. A) Comparison of the growth performance of the in silico iCH457 strain and a derivative strain of B. bacteriovorus 109 Davis on different carbon sources.
Experimental values of growth rate were calculated using the mass doubling time previously compiled in Ishiguro et al. [67]. The in silico growth rate was calculated with the minimal medium defined in S1 Text supplemented with the tested carbon source. B) Comparison of the biomass production predicted in silico with the available experimental data performed with the prey-independent B. bacteriovorus 109 Davis [67]. In vivo and in silico biomass data were expressed as Kendall’s rank correlation coefficient (τ = 0,88) for iCH457. GLU: glutamate, GLN: glutamine, PYR: pyruvate, LACT: lactate, SUCC: succinate. The statistically significant differences were calculated using two-way ANOVA followed by Bonferroni test. All comparisons were found non-significant (P<0.05).