Providing new insights on the biphasic lifestyle of the predatory bacterium Bdellovibrio bacteriovorus through genome-scale metabolic modeling
Fig 2
iCH457 metabolic model pipeline.
The draft of metabolic reconstruction was based on available metabolic models (iJN1411 and iJO1366), the genome sequence of B. bacteriovorus HD100 and the automatic model Seed. Manual curation is required to accurately fine-tune the information contained in the metabolic model and several steps of network validation and analysis are required to finally obtain the metabolic model iCH457. The general model iCH457 was constrained based on nutrient availability (minimal and rich in silico media), biological role (ATP production or biomass generation) and transcriptomic available data [41] for the generation of condition-specific models: iCHAP and iCHGP. GIM3E algorithm was used to construct the condition-specific models Rxns: reactions.