SynToxProfiler: An interactive analysis of drug combination synergy, toxicity and efficacy
Fig 1
A schematic overview of SynToxProfiler.
The dose-response data from drug combination screening, measured in both diseased (e.g. patient-derived cells) and healthy control cells (e.g. PBMCs), is provided as input to SynToxProfiler (left panel). Then, SynToxProfiler quantifies drug combination efficacy and synergy (using combination responses in diseased cells) as well as toxicity (using combination responses in control cells) for each drug pair (middle panel), and summarizes them into integrated synergy, toxicity and efficacy (STE) score. The increase in red color gradient in the efficacy and toxicity matrix (middle panel) denotes an increase in percentage inhibition of cancer and healthy cells, respectively. The color gradient from white to red in the synergy matrix shows the shift of drug interactions from antagonism to synergy. The STE score is further used to rank and visualize the drug pairs in 2D or 3D interactive plots (right panel). The color gradient from blue to red in the right panel depicts an increasing STE score from low to high.