Accurate prediction of kinase-substrate networks using knowledge graphs
Fig 7
Mass-spectrometry validation of a subset of LinkPhinder predicted phosphorylations.
A) Overview of the experimental design. B) Mass-spectrometry result: Specific LATS1 interactors and their phopshorylations. Bold rows indicate phosphorylation that were predicted by LinkPhinder. (*There is a risk that ZMYM2 binding might be unspecific. Some samples show high intensities in the GFP1 control, see panel D.) C) LinkPhinder predictions for the results in panel B. D) Mass-spec raw intensity values (dots) of the detected phosphorylation sites in GFP-LATS1 associated proteins under the indicated conditions (n = 6 replicates), and corresponding box plots indicating median (red line), upper and lower quartile (grey box), whiskers (most extreme values not defined as outliers), and outliers (plus marks) defined as values outside 1.5 times the interquartile range.