Accurate prediction of kinase-substrate networks using knowledge graphs
Fig 6
Experimental validation of model predictions.
A) HEK293 cells were transfected with non targeted siRNA (Scr) of the indicated siRNA against LATS1. Phosphorylation of CREB or p53 was measured using specific antibodies and normalised to the level of expression of the corresponding proteins. The graph shows the fold change of the phosphorylation of the specific residues with respect to the Scr control. B) HEK293 were transfected with empty vector (EV) or GAG-AKT or treated with AKTi IV (10μM) for 1 hour. Phosphorylated proteins were immunoprecipitated using an anti-AKT antibody and the immunoprecipitates were blotted with anti-MST2. The bars show the fold change with respect to the control. The experiments were repeated at least 2 times. Error bars represent standard variations.