Functional analysis of Rossmann-like domains reveals convergent evolution of topology and reaction pathways
Fig 9
Binding site divergence in RLM homologs.
RLM (SSEs colored rainbow, with yellow crossover loop) from homologs bind adenine nucleotide (magenta stick) and diphosphate (orange stick) components of NAD (black nicotinamide ring) from different substrates (stick, colored elements) using similar binding modes. NAD(P)-binding Rossmann-fold domain (slate cartoon) from (A) myo-inositol dehydrogenase (PDB: 4MIN) binds NAD and from (B) succinyl-CoA Synthetase (PDB: 2NU8) binds CoA (gray cysteamine). HUP domains (salmon cartoon) from (C) nicotinamide mononucleotide adenylyltransferase (NMNATase) (PDB: 1EJ2) binds NAD and from (D) bi-functional ATP Sulfurylase-APS Kinase (PDB: 2GKS) binds ADP.