Systems-level analysis of NalD mutation, a recurrent driver of rapid drug resistance in acute Pseudomonas aeruginosa infection
Fig 7
Validation of metabolic model using experimental growth data.
(A) Experimental growth curves of both sensitive (D-3rsw) and resistant (D+7bld) P. aeruginosa strains at various aztreonam concentrations (μg/mL). (B) Steady state distribution of biomass flux predicted from metabolic models for the same experimental conditions (except for D-3rsw at 4μg/mL aztreonam). PDF: probability density function. (C) Comparison of the measured growth rate (blue bars) with the model predictions (red bars). The measured growth rates were obtained by fitting an exponential growth model to the exponential phase of the growth curves shown in (A). The predicted growth rates were approximated from the mean of the biomass flux distributions shown in (B). The growth rates are relative to that of the sensitive strain in the absence of aztreonam (S0). Error bars: standard deviation.