Automated minute scale RNA-seq of pluripotent stem cell differentiation reveals early divergence of human and mouse gene expression kinetics
Fig 3
Dynamic genes in either mouse or human experiments also display earlier breakpoints in mouse compared to human cells.
(A) The distribution of all breakpoint times among 3721 dynamic genes in Mouse and 4332 dynamic genes in Human. The values indicate the number of breakpoints occurring prior to 60 minutes, 100 minutes, and 250 minutes. Some genes may have multiple breakpoints in each time-frame. B) Smoothed density plot as previously described are shown for all breakpoint times among mouse and human dynamic genes. The 99% confidence interval for the Wilcoxon rank sum statistic shift in distribution (ΔM) for breakpoint times in human versus mouse was 154 to 172 minutes. C) The percent of genes having an up or down trend immediately versus those displaying a delayed trend (a period of stable expression for five consecutive time-points in mouse cells or three consecutive time-points in human cells followed by an up or down response). The 99% confidence interval for the difference in the percent (ΔP%) of genes having an immediate up/down trend was -3.89% to -0.89%. D) The number of genes that had monotonic expression patterns (i.e. no breakpoints) along the time course trending either up or down. The 99% confidence interval for the difference in the percent (ΔP%) of monotonic genes was 25.5% to 30.9%.