Network-based analysis of prostate cancer cell lines reveals novel marker gene candidates associated with radioresistance and patient relapse
Fig 6
Experimental validation of VGF as regulator of cell radioresistance.
(a) Increased VGF expression in radioresistant DU145 and LNCaP in comparison to their radiosensitive parental cell lines in our microarray data. Three biological replicates were considered for each condition. (b-d) RT-qPCR analysis of VGF expression under different conditions. (b) Increased VGF expression in four independent radioresistant DU145 and three independent radioresistant LNCaP clones relative to their radiosensitive parental cell lines. (c) Increased VGF expression in sphere relative to monolayer cultures of parental DU145 and LNCaP cells. (d) Reduced VGF expression in parental DU145 and LNCaP cells induced by siRNA-mediated gene silencing relative to negative controls. (e-f) Increased radiosensitivity of parental DU145 and LNCaP cells induced by siRNA mediated reduction of VGF expression. Shown are average fractions of surviving cells in log10-scale for increasing radiation dose. Error bars represent the standard error of the mean and ‘n’ specifies the number of biological replicates. Corresponding linear-quadratic (LQ) model curves are shown in S11 Fig.