Molecular mechanisms of fentanyl mediated β-arrestin biased signaling
Fig 6
Fentanyl derivatives with no β-arrestin signaling.
a-b, Structure based rational and chemical structure of newly synthesized fentanyl derivatives. c, Gi complex and β-arrestin coupling dose-response curve for DAMGO, fentanyl, and compounds FD1-3 measured by nanoBiT direction interaction assays. EC50 and maximal coupling efficacy compared to DAMGO are shown. Values and error bars reflect mean ± s.e.m. normalized to DAMGO of three technical replicates.