Molecular mechanisms of fentanyl mediated β-arrestin biased signaling
Fig 5
Effects of M153 mutations on ligand induced β-arrestin and Gi complex coupling.
a, Side-by-side structural comparison of orthosteric site interactions between DAMGO (PDB code: 6DDE) and fentanyl bound μOR. b-c, Dose-response curve for DAMGO and fentanyl induced Gi complex and β-arrestin coupling for wildtype and M1533.36 mutant receptors measured by nanoBiT direct interaction assays. EC50 and maximal coupling efficacy compared to DAMGO are shown. Values and error bars reflect mean ± s.e.m. normalized to DAMGO of three technical replicates.