From Escherichia coli mutant 13C labeling data to a core kinetic model: A kinetic model parameterization pipeline
Fig 1
Pictorial representation of the kinetic parameterization pipeline for constructing kinetic models of metabolic networks.
(1A): A set of isotopic labeling data across a range of genetic and/or environmental conditions must be generated or procured. (1B): A stoichiometric model must be constructed. (2): 13C-MFA is performed, and flux ranges are elucidated using the procured isotopic labeling data across all strains from step 1A and the stoichiometric model constructed in step 1B. (3): The flux distributions that were generated in step 2 are used as training data for parameterizing the kinetic model using the stoichiometric model constructed in step 1B and the K-FIT algorithm.