p13CMFA: Parsimonious 13C metabolic flux analysis
Fig 3
Schematic representation of the central carbon metabolism flux map obtained with p13CMFA in the HUVECs case study.
Reaction fluxes are indicated for some key reactions in μmol·h-1·million-cells-1. Arrows indicate net flux direction, and line width is representative of flux magnitude. Reactions and metabolites of redox and energy metabolism have been omitted from this figure for clarity. 2PG: 2-Phosphoglycerate. 3PG: 3-Phosphoglycerate. AcCoA: Acetyl-CoA. aKG: α-Ketoglutarate. Asp: Aspartate. bPG13: 1,3-Bisphosphoglycerate. Cit: Citrate. DhaP: Dihydroxyacetone phosphate. Fru16bP: Fructose 1,6-bisphosphate. Fru6P: Fructose 6-phosphate. Fum: Fumarate. GaP: Glyceraldehyde-3-Phosphate. Glc: Glucose. Glc1P: Glucose 1-phosphate. Glc6P: Glucose 6-phosphate. Gln: Glutamine. Glu: Glutamate. Glucon6P: Gluconate 6-phosphate. Glygn: Glycogen. iCit: Isocitrate. Lac: Lactate. Mal: Malate. OAA: Oxaloacetate. PEP: Phosphoenolpyruvate. Pyr: Pyruvate. Rib5P: Ribose 5-phosphate. Rul5P: Ribulose 5-phosphate. Sed7P: Sedoheptulose 7-phosphate. Suc: Succinate. SucCoa: Succinyl-CoA. UDPGlc: Uridine diphosphate glucose. The subscripts e, c, and m denote the extracellular, cytosolic and mitochondrial compartments, respectively.