p13CMFA: Parsimonious 13C metabolic flux analysis
Fig 2
Flux spectrum, GIMME solutions, 13C MFA flux ranges, and p13CMFA solutions for some key reaction fluxes in the HUVECs case study.
Flux spectrum represents the feasible flux ranges considering only the stoichiometric and thermodynamic constraints and the measured extracellular fluxes. GIMME flux values are obtained when total reaction flux is minimized weighted by gene expression without integrating 13C data. For 13C MFA, the flux values obtained after the 13C MFA optimization and the range of the 95% confidence intervals for such values are shown. The p13CMFA flux values are obtained when total reaction flux is minimized within the 13C MFA solution space. Fluxes are expressed in μmol·h-1·million-cells-1.