On the optimal design of metabolic RNA labeling experiments
Fig 3
Estimates for pulse-chase SLAM-seq data [12].
Degradation rates and 95% confidence intervals are shown for different chase time points. For short chase times, the majority of genes have poorly identified degradation rates (see subsets [0, 0.5], [0, 3], [0, 6] hr). On the other hand, longer chase times do not allow to precisely estimate rates for unstable genes ([0, 12] hr).