Interkinetic nuclear movements promote apical expansion in pseudostratified epithelia at the expense of apicobasal elongation
Fig 7
Apicobasal elongation requires a specific elongation force.
Simulations with contractile apical springs, normal INM and high noise. A, apicobasal length of the PSE (AB) and mean nuclear position along the AB axis (N) over time expressed in micrometers (see S5 Movie). B, Number of pseudolayers of nuclei along the AB axis. C, straightness of apical domain (net distance between the first and last apical point divided by the actual distance between these two points). D-G, net width of apical (magenta), nuclear (black) and basal (cyan) domains of the PSE with dynamic apical-apical springs over time with 50 (D), 40 (E) and 30% (F-G) of daughter cells being excluded from the 2D plane. For each domain, the distance between the first and last point along the lateral axis is computed and its evolution plotted over time. D-F, slow update of basal point positions (as in all previous Figs). G, fast update of basal point positions (twice faster). Each simulation was performed over 480 iterations (48h of biological time) for 10 repetitions. Each curve represents the mean value of each dataset for the parameter plotted.