Interkinetic nuclear movements promote apical expansion in pseudostratified epithelia at the expense of apicobasal elongation
Fig 6
Mean volume and mean apical surface of chick neuroepithelial cells decreases between 2 and 3 days of development.
A, Diagram depicting the regions used for the preparation of cell suspensions and open book histology from chicken embryos at 2, 3 and 4 days of development (see Methods for details of the experimental procedures). Region monitored (from somite 15 to 20, forelimb region) is indicated by a dotted line. B, representative images of neuroepithelial cells in suspension after neural tube dissection and enzymatic dissociation. C, mean volume of neuroepithelial cells over time (n48h = 82; n72h = 568; n96h = 1168). Cells get significantly smaller from 2 to 3 days of development and remain stable. Box and whiskers plot: the box extends from the 25th to the 75th percentile; the whiskers show the extent of the whole dataset. The median is plotted as a line inside the box. One-way ANOVA (Kruskal-Wallis) followed by Dunn’s multiple comparisons. ****, p<0.0001; ns, p>0.9999. D, en face view of the apical domain of the intermediate region of the neuroepithelium (actin is stained by Phalloidin). E, mean area of the individual apical surfaces over time (n48h = 67; n72h = 66; n96h = 81). Apical surfaces shrink from 2 to 3 days of development and remain stable. Dots represent mean of the dataset, error bars represent S.D. One-way ANOVA followed by multiple comparisons. ****, p<0.0001; ns, p = 0.1586.